Nagalase Testing (Red Labs Belgium Europe)

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Nagalase Activity Assay

Vitamin K

Vitamin K2 (also known as Menaquinone) is recently calling the interests of many researchers and health practitioners, especially in addition to vitamin D3. There are several health benefits associated to proper vitmin K2 levels. It’s main function is the transportation of calcium to the right areas in the body. Doctors have long known that people with a lack of calcium in their bones are more likely to possess an excess of calcium in their arteries, and vice versa. The resulting lack of calcium in bone leads to osteoporosis, while the deposition of calcium in the arterial wall leads to coronary heart disease and other manifestations of cardiovascular, renal, and neurodegenerative disease. This shows there is actually is a strong connection between these two conditions (as unconnected the might seem).
Some 85 years ago, biochemist in Copenhagen discovered the 2 body’s naturally occurring vitamin K’s: vitamin K1 (phylloquinone) and vitamin K2 (menaquinone), both fat-soluble but with different properties and characteristics.
Vitamin K1:

• Fat- soluble.
• Main function is healthy blood-clotting (activating coagulation factors in the blood).
• High amounts found in green leafy vegetables.
• Low total absorption (only +- 10%).
• Stored in the liver.

Vitamin K2:

• Fat-soluble
• Main function is to transport calcium to the places it needs to be (bones) and away from places it shouldn’t be (soft tissues). Also has blood-clotting properties.
• Mainly made in your gut under influence of a bacteria present in fermented foods. MK-7 (long chain) found in natto (fermented soy) and other fermented vegetables. Low amounts MK-4 (short chain form) found in butter, eggs, cheese and organ meats.
• Almost complete absorption (+- 90%).
• Also goes to the liver but redirected to important body parts.
• Several proteins depend on K2 for activation.

This differences show that it is easy to consume enough vitamin K1 trough diet but that this vitamin is broken down pretty fast (only about 10% reaches circulation) and is not as essential as vitamin K2, which has blood-clotting properties too. It is harder to consume enough vitamin K2 through your diet (especially for vegans) but there is a much higher absorption rate than with vitamin K1.

There are proteins in the body that are dependent on vitamin K2, without it they remain inactivated. The first discovered, Bone Morphogenetic Protein-2, is such a protein. Vitamin K2 is necessary for the manufacturing of blood-clotting proteins in the liver. The next two proteins are considered the most important proteins activated by vitamin K2;
Osteocalcin
Osteocalcin is a protein activated by vitamin K. This protein is found in bone and teeth. Its main function is bone building. It also acts as a hormone in the body, causing beta cells in the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin.

Matrix Gla Protein
Matrix Gla Protein is another protein dependent on the presence of vitamin K. This protein inhibits vascular mineralization and binds with calcium ions. MGP is found in bone, cartilage, heart, and kidney and its production is increased by vitamin D.

According to Professor Cees Vermeer, one of the world’s top vitamin K2 researchers:

“The only mechanism for arteries to protect themselves from calcification is via the vitamin K-dependent protein MGP. MGP is the most powerful inhibitor of soft tissue calcification presently known, but non-supplemented healthy adults are insufficient in vitamin K to a level that 30 percent of their MGP is synthesized in an inactive form. So, protection against cardiovascular calcification is only 70 percent in the young, healthy population, and this figure decreases at increasing age.”

Here is why vitamin K2 is so important for cardiovascular health; because MGP stops calcification and could even regress arterial calcification induced by vitamin K deficiency.

Vitamin D3 and vitamin K2: the correlation
Vitamin D is naturally obtained after sunlight is synthesized through your skin. However many people don’t get enough sunshine in modern life and need to supplement vitamin D3.
Vitamin D provides improved bone development by helping you absorb calcium, but vitamin K2 directs the calcium to your skeleton, while preventing it from being deposited where you don’t want it (organs, joint spaces and arteries). Meanwhile vitamin D3 stimulates the MGP production the vitamin K is needed to activate the MGP (the Matrix Gla Protein) to actually get it functioning. Thus these 2 vitamins correlate with each other.

Vitamin K deficiency
Vitamin K deficiency can be caused by the use of antibiotics which destroy the colonic bacterial synthesis of the vitamin. Also fat mal-absorption can be the cause of a deficiency (for example with celiac disease).
In a small study done in Japan investigators found that this nutrient dramatically reduced the risk of liver cancer.

“40 women who had liver cirrhosis from viral infections, there was a marked difference in
the incidence of liver cancer, with only 2 of 21 developing cancer in the MK-4 group
compared with 9 of 19 in a control group.”

– Habu D, Shiomi S, Tamori A, et al. Role of vitamin K2 in the development of hepatocellular carcinoma in women with viral cirrhosis of the liver. JAMA. 2004 Jul 21;292(3):358-61.

In another study it showed that 61 people recovering from surgical removal of hepatocellular carcinoma (liver tumor) using 45mcg of vitamin K2 daily it enhanced the cancer-free survival rate.
In the laboratory (in vitro), vitamin K2 demonstrates inhibitory effects against myeloma and lymphoma, suggesting possible applications for individuals fighting these hematologic cancers.

Vitamin K is a fat-soluble vitamin and therefore it needs fat to be absorbed by the body (as does vitamin D).

• Excessive intake of vitamin K leading to toxicity is uncommon and hasn’t been reported so far.

I have copied the following from Dr. Mercola’s website:

Calcium and Vitamin D Without Vitamin K2 May Be Dangerous

If you take calcium and vitamin D but are deficient in vitamin K2, you could be worse off than if you were not taking those supplements at all, as demonstrated by one meta-analysis that linked calcium supplements to heart attacks.
This meta-analysis looked at studies involving people taking calcium in isolation, without complementary nutrients like magnesium, vitamin D and vitamin K2, which help keep your body in balance.
In the absence of those other important cofactors, calcium can have adverse effects, such as building up in coronary arteries, initiating excessive clotting and causing heart attacks, which is really what this analysis detected. So if you are going to take calcium, you need to be sure you have balanced it out with vitamin D and vitamin K2.

Food	Vitamin K2
Natto (3.5 ounces)	1,000 mcg
Fermented vegetables made with Kinetic Culture (2 ounces)	400-500 mcg
Whole egg mayonnaise	197 mcg
Gouda or Brie cheese (1 ounce)	75 mcg
Miso	10-30 mcg
Lamb or duck (1 cup)	6 mcg
Beef liver (1 cup)	5 mcg                                    Bacillus subtilis
Dark meat turkey (1 cup)	5 mcg
Chicken liver (1 cup)	3 mcg

Also keep in mind that fermented foods contain a wide variety of different bacteria, and only certain ones—such as Bacillus subtilis — actually make vitamin K2.

The different MK’s
There are several different forms of vitamin K2. MK-8 and MK-9 come primarily from fermented dairy products, like cheese. MK-4 and MK-7 are the two most significant forms of K2 and act very differently in your body.
MK-7, which is the form used in the featured study, is a newer agent with more practical applications because it stays in your body longer; its half-life is three days, meaning you have a much better chance of building up a consistent blood level, compared to MK-4 or vitamin K1.
Dr. Vermeer recommends between 45 mcg and 185 mcg daily for adults. You must use caution on the higher doses if you take anticoagulants, but if you are generally healthy and not on these types of medications, opt for 150 mcg a day.

Sources:
http://articles.mercola.com/sites/articles/archive/2015/01/11/vitamin-k1-k2.aspx
http://articles.mercola.com/sites/articles/archive/2013/10/19/vitamin-d-vitamin-k2.aspx

Menaquinone-7
So we can now conclude that MK-7 is the superior form of vitamin K.
All K vitamins are similar in structure, but differ in the length of the side chain. The longer the side chain, the better the effect and efficiency. The long-chain menaquinones (especially MK-7) are the most desirable as they are nearly completely absorbed (and thus the body requires smaller doses) and stay in the blood for the longest time.
Meaning, vitamin K2 is also available for tissues outside the liver, namely bones, arteries and soft tissues.
There are manufactures out there today that have managed to synthesize a nature-identical form of MK-7.
One of those manufactures is MenaQ7. Quoted from their website: “The trans form fits perfectly into the 3D structure of the carboxylase enzyme, enabling activation of the vitamin K dependent proteins.”

Health benefits from vitamin K2:
• Directs calcium into the bones and out of the arteries = Strong bones and teeth
• Reduces arterial calcification = Improved elasticity of arteries and veins
• Improves the nervous system
• Decreases muscles cramps
• Improves insulin resistance

Conclusion:
• Vitamin K is a very important vitamin, especially when supplementing vitamin D3.
• Aspire to obtain your vitamin K through your diet when obtaining your vitamin D3 through sunlight or low dose supplementing. A filtered, good grade cod-liver oil contains small amounts of vitamin D and K.
• Definitely supplement vitamin K2 when supplementing vitamin D3 with an illness (i.e. high doses of vitamin D3).
• Definitely supplement vitamin K2 when supplementing calcium!
• Supplement vitamin K2 when suffering from cardiovascular disease.
• Opt for a MK-7 supplement (from natto).
• Take somewhere between 100mcg – 200mcg of vitamin K2 daily, depending on your illness, vitamin D3 supplementation and diet.
• Vitamin K can be beneficial for the prevention and treatment of liver cancer (and possible other cancer too).

Other sources:
http://www.lifeextension.com/magazine/2008/3/Protecting-Bone-And-Arterial-Health-With-Vitamin-K2/Page-01

General Guideline – Gc-protein products

General Guideline – Gc-protein products   

                    

 

  • 2,500ng MAF transport protein Spray

This is the low strength spray which delivers approximately 25ng of VDB Protein per spray.
The sprays are designed for sublingual administration (to spray under the tongue) and will be absorbed by the mucous membranes under the tongue. These membranes can very readily absorb the protein from the saline and thus this is an easy way of taking VDBP (or GcMAF) and available to everyone. Another advantage of the spray is that the whole family (including children) can benefit from this medicine as there is usually more than one family member with a suppressed immune system.

Why the 2,500ng spray?
In some cases it might be better to start off on a low dosage and gradually increase the amount of sprays per dosage.
It can be used for non-life-threatening diseases and because of its low strength it’s suitable for children with health conditions.

To see product visit: https://firstproengineering.com/products/detail/PRO_00005
• 15,000ng MAF transport protein Spray
This is the high strength spray which delivers approximately 150ng of VDB Protein per spray.
This spray is, just as the 2,500ng spray, designed for sublingual administration and will be absorbed in the same way.

Why the 15,000ng spray?
The 15,000ng contains a significant higher amount of Gc-protein per serving and is therefore more suitable for more serious health conditions.
Some advice to go low and slow, but depending on the gravity of the condition a higher strength can be sensible.
This spray is not advised for autism as there have been side effects reported due to an “over” detoxification process after taking the high strength products.
This spray too can be used next to other treatments and is often advised together with one of the injectable GcMAF.
This spray is a good alternative if you cannot or do not want to inject GcMAF.

To see product visit: https://firstproengineering.com/products/detail/PRO_00004

 

• Pure Emu oil with 5000ng MAF transport protein
This product is in general used to rub on your veins or lymph nodes and absorbed through the skin.
Although GcMAF mainly works from the inside out (activates the immune system which than attacks the problem) this product can give a relieve to affected skin and painful joints. Rub gently onto the skin and leave for at least 20 minutes.
Emu oil is both non-comedogenic (does not block pores) and easily absorbed by the skin.
When massaged into the skin Emu oil is quickly penetrates and saturates all the underlying tissues. Its action moisturizes the skin and provides anti-inflammatory effects to muscles and joints. Emu Oil is bacterio-static, so bacteria will not grow in it and therefore lasts a very long time. It is non-allergenic and yet no side effects have ever been reported.

Why the MAF Emu Oil?
This product is mainly used alongside one of the other MAF products. For example, on its own it can topically be used for: skin conditions, swollen lymph nodes, joint pains, inflamed body parts etc.
Also, this is a good option if the 2,500ng spray is too low for you but the 15,000ng spray is too high. Apply to your wrist or neck vein and allow 30 min to absorb (so do not wash your hands in this time span just to be sure the entire product is absorbed). Children with skin problems can safely use the MAF Emu Oil on its own.

To see product visit: https://firstproengineering.com/products/detail/PRO_00006

 

• Injectable 1,500ng MAF transport protein
This is the low strength vial for intramuscular injection (in the muscle).
It contains 2.5 ml with 1,500ng MAF protein in total.
An average dose contains 0.20 to 0.25 ml. This means each dose delivers approximately 120ng – 150ng per injection. Most people take 1 injection every 3 days, or 2 injections a week (e.g. Monday and Friday) which would mean this vial can last somewhere between 1 month and 1,5 month.
An advantage of injecting is that you directly deliver the protein into the muscles where it will be absorbed by one of the many capillaries and brought directly to your bloodstream. There will be no loss of protein what so ever.
Make sure you know where and how to inject or that you have someone that can inject it for you.
We advise you purchase 1 ml syringes. Generally this needle sizes are applicable:
– normal legs: 0,7 x 32 mm
– bigger legs: 0,8 x 38 mm
– very thin legs: 0,6 x 25 mm
Make sure you have some disinfectant to use on your skin.

Why the 1,500ng vial?
This GC-protein strength is advised for people with chronic and continuous (but not severe) conditions.
With some conditions you can either use the low or high strength products depending on the severity of your symptoms.
If your symptoms aren’t fairly severe the 1,500ng Gc-Protein will be sufficient.

To see product visit: https://firstproengineering.com/products/detail/PRO_00002

 

• Injectable 7,500ng MAF transport protein
This is the high strength vial for intramuscular injection (in the muscle).
It contains 2.5 ml with 7,500ng MAF protein in total.
An average dose contains 0.20 to 0.25 ml (although cancer patients are advised to go up to 0.5 ml per injection). This means it delivers approximately 600ng – 750ng per injection (1500ng with 0.5ml).

Why the 7,500ng vial?
This injectable is currently the most used strength because it is advised for more serious conditions. If you suffer from a life-threatening, chronic or severe and continuous condition the 7,500ng Gc-Protein will be sufficient.

To see product visit: https://firstproengineering.com/products/detail/PRO_00003

 

Before choosing what product would serve you the best, decide whether your condition is:

– Serious, life threatening
– Persistent, continuous and chronic
– Non-life-threatening

In some conditions combinations are advised. For an “example protocol” please click here

This information is meant as a general guideline. For a personal protocol please discuss with your health practitioner.

 

Disclaimer
The FDA has not evaluated these statements. This is not intended to diagnose or treat any disease. All information does not replace advice of your health care provider MD/Dr.

Side Effects

                          Side Effects

Possible side effects that can occur when using any GcMAF products.
It’s important to note that the Vitamin D Binding Protein (GcMAF or MAF protein) occurs naturally in the body it has no side effects by itself, any observed effects are those created by an activated immune system.

Supplementing vitamin D3

Vitamin D3 is produced by the body if exposed to enough sunshine. It is a potent immune modulator, making it very important for the prevention of cancer, bone and teeth deterioration, mental problems and immune related diseases.

Exercise and the immune system

How does exercise affect the immune system?
When you exercise regularly, there are a number of health benefits that come with it. Your heart gets stronger and is able to pump more blood throughout your body when you exercise. Your lungs get better equipped at handling oxygen and providing it to your organs and to the rest of your body (and remember that cancer hates oxygen!). Your muscles also get stronger as you use them more often. And very important: your lymph system gets moving. Doctors have found that exercise can boost your immune system by providing a boost to the cells in your body that are assigned to attack bacteria. These cells appear to work more slowly in people who don’t exercise than in those that do. As a result, if you exercise, your immune system is better equipped to handle pathogens that could cause you to become sick, or boost your immune system if you already have an illness.

Why is Nagalase important?

1. Nagalase causes immunodeficiency. Nagalase blocks production of MAF, thus preventing the immune system from doing its job. Without an active immune system, cancer and viral infections can grow unchecked.
2. As an extremely sensitive marker for all cancers, Nagalase provides a powerful system for early detection.
3. Serial Nagalase testing provides a reliable and accurate method for tracking the results of any therapeutic regimen for cancer, AIDS, or other chronic viral infection.

Using Nagalase to screen for cancer & viruses

In the (hopefully not-too-distant) future, once Nagalase testing and GcMAF are available, all people at risk of cancer—i.e., everyone over the age of 40—will get an annual Nagalase test along with their other routine blood tests (complete blood count, comprehensive metabolic panel, vitamin D, lipid panel, etc.).

Nagalase is remarkably efficient

Because it is an enzyme—a catalyst—Nagalase performs this malicious ritual over and over and over again, and each time it comes away unscathed and unchanged. One Nagalase molecule can thus destroy a huge quantity of Vitamin D Binding Protein molecules.

Using Nagalase to track effectiveness of cancer & viral therapy

The more cancer cells present in the body, the more Nagalase they generate. Thus, Nagalase is the perfect marker for determining response to cancer therapies because, in a given patient, tumour burden will always be proportional to the Nagalase level.